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KMID : 0665420180330040337
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Korean Journal of Food Culture
2018 Volume.33 No. 4 p.337 ~ p.344
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Effect of Germinated Brown Rice on LPS-Induced Inflammation in Adipocytes
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Park Mi-Young
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Abstract
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Germinated brown rice (GBR, Orysa sartiva L.) has been reported to have anti-obesity and anti-inflammatory effects.
However, the mechanisms underlying these effects in adipocytes are not fully understood. Therefore, this study was
conducted to explore the anti-inflammatory mechanisms of GBR on lipopolysaccharide (LPS)-stimulated 3T3-L1
adipocytes. 3T3-L1 adipocytes were pretreated with GBR extracts (0-20 mg/mL) 1 h before LPS stimulation. The mRNA
expression of adipokines and Toll-like receptor 4 (TLR4) were measured by RT-PCR. The protein expressions of TLR4-
related molecules were detected by western blotting and nuclear factor-κB (NF-κB) activation was measured. Our results
showed that GBR extract dose-dependently inhibited mRNA expression of LPS-induced tumor necrosis factor-α (TNF-α),
interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1). GBR extract was found to inhibit LPS-induced
mRNA expression of TLR4 and protein expression of both myeloid differentiation factor 88 (MyD88) and TNF receptorassociated factor 6 (TRAF6). Furthermore, GBR extract significantly inhibited extracellular receptor-activated kinase (ERK) phosphorylation and NF-κB activation. These results suggest that GBR extract has the anti-inflammatory effects on LPSinduced inflammation via inhibition of TLR4 signaling, includingthe ERK and NF-κB signaling pathways, in adipocytes.
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KEYWORD
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Orysa sartiva L. , Inflammation , Germinated brown rice , Toll-like receptor 4
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